Patterns of lipid changes with growth of chick sciatic nerve Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/pc289n76k

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  • Lipid changes with growth of chick sciatic nerve
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  • Phospholipids and cerebrosides of the normal chick sciatic nerve were extracted by Folch procedures and separated with silica gel thin-layer chromatography using various chloroform-methanol solvents. The subsequent chemical assays were expressed as a function of the developmental stages of the chick from 15 days of incubation to 7 days after hatching (28 days from onset of incubation). A histological study of chick sciatic nerves at ages 18, 20, 22 and 25 days was conducted to identify the period of myelination. Luxol Fast Blue G stain was used to identify the myelin. Myelin, is poorly identified at 18 days but notably present at 25 days. The assays of the phospholipids, expressed as relative percent of the total phospholipid phosphorus, indicated that ethanolamine-phosphoglyceride ratio increases significantly from 17 days to 23 days from onset. Sphingomyelin ratio also increased during this time, but not as much. While the relative amount of serinephosphoglyceride remained constant and cholinephosphoglyceride diminished. In vivo introduction of inorganic ³²P was used to follow the metabolism of the phospholipids. The period of most active uptake of this substrate was also from 17 to 23 days from onset. The substrate failed to be absorbed well just prior to hatching. This was possibly due to inactivation of the allantoic circulation responsible for assimilation of the substrate prior to hatching. In the sciatic nerve of the 16 day chick embryo there is 3μg of cerebroside per mg of wet weight of tissue. As development takes place, this component increases to 26 μg per mg wet weight of tissue at 27 days from onset. Cerebroside and phospholipid metabolism was followed with in vivo injection of galactose-1-¹⁴C. At the earlier ages, the substrate was metabolized predominantly into non-lipid components. But from 20 to 28 days, the radioactivity was recovered increasingly from the lipid fractions of the tissue. Proteolipid protein in the lipid extract was also determined. It was found in this tissue at all ages, but only after hatching is there a notable increase in its amount. Implications of these lipid changes as coincident to myelination in the peripheral nerves were discussed.
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