Solubility and electophoretic studies of the protein deposited during oocyte development in Leucophaea maderae (Fab.) Public Deposited

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  • During the maturation of the ovaries of Leucophaea maderae (Fab.), the terminal oocytes increase in length from 1.00 mm to greater than 5.00 mm. This increase in length is accompanied by approximately a 70-fold increase in protein content. The relationship between the ovarial size and total protein indicated that the protein is added in a continuous manner during the maturation of the ovary. Solubility characteristics of the protein were examined and 80% or more of the protein was found to be soluble at conditions of high ionic strength (0.4 or greater) or conditions of high pH (9.4 or greater). Electrophoresis on 7.5% polyacrylamide gels of nearly mature ovaries dissolved in 0.4 M sodium chloride produced six bands. When electrophoresis was carried out on a series of different sized ovaries during maturation, the major change in the protein pattern was found to be the appearance and increase of Band III. This band constitutes the major portion of the proteins in the mature ovary and stains with Sudan black B which indicates that it is a lipoprotein. Further solubility studies indicated that the major protein which was deposited during growth is not soluble in 0.2 M sodium chloride. Two of the minor bands deposited during maturation and other proteins which resemble the ovarial proteins of the immature oocyte are soluble in 0.2 M sodium chloride.
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