Some aspects of an oxidative tryptophan decarboxylating enzyme in Pisum sativum Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/pv63g389w

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  • Three types of experiments were designed to further characterize the tryptophan decarboxylating enzyme of Reed and Crecelius (1967). 1) Light inhibition studies were made with etiolated pea seedlings to determine the in vitro response of the tryptophan decarboxylating enzyme. Assays of indoleacetic acid oxidase (IAA oxidase) and guaiacol-active pea seedling peroxidase were included for comparison. The studies showed that IAA oxidase and the tryptophan decarboxylating enzyme had essentially the same inhibition response caused by constant exposure of etiolated pea seedlings to light. The guaiacol-active peroxidase was not inhibited. 2) Sephadex chromatography was performed to ascertain any differences in the elution of the three enzymes. A large portion of the tryptophan decarboxylating enzyme activity was retarded by the column. The activity peaks of the other enzymes assayed, IAA oxidase and pea seedling peroxidase, coincided with the main protein peak. 3) A study was performed with catechol to determine the type of inhibition caused by polyphenolic compounds. The results indicate that the inhibition was competitive. A survey of polyphenolic compounds was conducted to determine their relative efficiency of inhibition of the tryptophan decarboxylating enzyme. All of the compounds tested were found to be very potent inhibitors of the tryptophan decarboxylating enzyme.
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