Graduate Thesis Or Dissertation

 

Building a bridge from chromosomes to microtubules in Neurospora crassa Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/pv63g492d

Descriptions

Attribute NameValues
Creator
Abstract
  • DNA is often described as the “blueprint for life”. In eukaryotes this information is contained within linear chromosomes of varying size and number. During cell division, the chromosomes must be faithfully segregated into each daughter cell to avoid disease and sustain life. This process is carried out by the kinetochore complex, linking each chromosome to the spindle microtubules during anaphase. Advances over the past several decades have shed light on the characteristics of centromeric DNA sequence, as well as the mechanisms by which the proteins of the kinetochore recognize this locus and build a bridge to microtubules. However, the extent of sequence variability within the centromere has only recently become clear, requiring us yet again to address questions about the role of DNA sequence in centromere function. Divergence also is a hallmark of kinetochore proteins. Examination of the centromeres and kinetochores of a more diverse array of organisms should provide insights into the conserved sequence elements and protein interactions that are fundamental to chromosome segregation. In this dissertation, I describe extensive characterization of the centromere sequence of numerous N. crassa strains, revealing a large degree of variability but no observed functional differences. This work provides insight into the extent, and mechanisms, of centromere sequence evolution. I also describe studies of the kinetochore proteins of N. crassa, including a full set of tools for extensively probing the inner workings of the kinetochore of this filamentous fungus. This work emphasizes the extent of variability in this critical protein complex and suggests the need for similar work in more diverse organisms.
License
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Rights Statement
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Submitted by Steven Friedman (friedmst@oregonstate.edu) on 2017-07-07T17:39:10ZNo. of bitstreams: 1FriedmanStevenA2018.pdf: 87190675 bytes, checksum: 320532e1cbd4463a7c4b8352d07e575e (MD5)
  • description.provenance : Approved for entry into archive by Darcy Miller(darcy.miller@oregonstate.edu) on 2017-07-20T18:46:07Z (GMT) No. of bitstreams: 1FriedmanStevenA2018.pdf: 87190675 bytes, checksum: 320532e1cbd4463a7c4b8352d07e575e (MD5)
  • description.provenance : Rejected by Julie Kurtz(julie.kurtz@oregonstate.edu), reason: Hi Steven,Thanks for uploading to ScholarsArchive. I am rejecting your dissertation to change the commencement date on the bottom of the title page to read - Commencement June 2018Everything else looks good. Once revised, log back into ScholarsArchive and go to the upload page. Replace the attached file with the revised PDF and resubmit.Thanks,Julie on 2017-07-03T21:29:57Z (GMT)
  • description.provenance : Submitted by Steven Friedman (friedmst@oregonstate.edu) on 2017-06-29T15:13:16ZNo. of bitstreams: 1FriedmanStevenA2017.pdf: 87193628 bytes, checksum: d1902c0ec67102e37c8447a601b3c60f (MD5)
  • description.provenance : Made available in DSpace on 2017-08-09T18:26:03Z (GMT). No. of bitstreams: 1FriedmanStevenA2018.pdf: 87190675 bytes, checksum: 320532e1cbd4463a7c4b8352d07e575e (MD5) Previous issue date: 2017-05-31
  • description.provenance : Approved for entry into archive by Steven Van Tuyl(steve.vantuyl@oregonstate.edu) on 2017-08-09T18:26:03Z (GMT) No. of bitstreams: 1FriedmanStevenA2018.pdf: 87190675 bytes, checksum: 320532e1cbd4463a7c4b8352d07e575e (MD5)
Embargo reason
  • Existing Confidentiality Agreement
Embargo date range
  • 2017-11-08 to 2019-08-10

Relationships

Parents:

This work has no parents.

In Collection:

Items