Graduate Thesis Or Dissertation
 

Vascular and extravascular fluid volumes in the Pacific hagfish, Eptatretus stoutii (Lockington)

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  • The partitioning of body fluids in the Pacific hagfish was investigated using several experimental techniques. Direct measurement of the blood volume was obtained through the simultaneous use of tagged red blood cells and plasma proteins labeled with Evan's blue dye. Blood cells obtained from donor animals were incubated with L-methionin-methyl-C¹⁴ in a physiological saline for two hours. The labeled cells were washed three times and injected into either the frontal or caudal area of the subcutaneous sinus of experimental animals. Blood samples were collected over a period of 104 hours through an indwelling catheter in the posterior portion of the subcutaneous sinus. Dilution curves were obtained. Fluid volume calculations were based upon values extrapolated to "zero" time. The packed blood cell volume for five animals was 4.9 ± 2.0 percent of the body weight. The plasma volume was 13.8 ± 3.1 percent of the body weight. A second group of experimental animals was used to estimate the extracellular fluid volume, as measured by the dilution of inulin-carboxyl-C¹⁴. The average of 25.9 ± 5.1 percent of the body weight was obtained. The interstitial fluid (lymph) being the difference between the extracellular fluid volume and the total blood volume was 7.2 percent of the body weight. Total body water was determined following the dehydration of individual animals to a constant weight of 104 ± 1°C. The value for five animals was 74.6 ± 3.4 percent of the body weight.
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