Graduate Thesis Or Dissertation

 

Simple sequence repeat marker development and fingerprinting in Cuphea lanceolata Ait. and C. viscosissima Jacq Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/q811kp32v

Descriptions

Attribute NameValues
Creator
Abstract
  • Microsatellites or simple sequence repeats (SSRs) are highthroughput sequence based DNA markers that have been exploited as tools for measuring genetic distance and diversity in evolutionary studies, protection of intellectual property, genetic resource conservation management, cultivar identification and molecular breeding of crop plants. We isolated and sequenced 1095 clones from genomic libraries enriched for CA, GA and GAA repeats. Unique SSRs were identified in 425 clones and primers were designed for 271 SSR sequences. Of the latter, 184 primer pairs produced clean SSR markers. Twenty nine functional SSR markers were used for genetic diversity study on 94 accessions of Cuphea. The Cuphea genotypes in the study panel included 20 accessions of C. lanceolata and 74 accessions of C.viscosissima. The mean number of alleles for C. lanceolata and C. viscosissima were 10.97 and 4.76 respectively. Similarly, markers amplifying C. lanceolata had a higher mean polymorphic information content (PlC) score (0.74) as compared to C. viscosissima (0.42). In C. lanceolata, a positive correlation was observed between maximum repeat count (MRC) and number of alleles (r=0.40) as well as MRC and standard deviation of molecular weight (SDMw) (r=0.72). C. viscosissima demonstrated a similar trend, albeit with a greater magnitude of correlation, between MRC and number of alleles (r=0.70) and MRC and SDMw (r=0.78). The SSR markers described herein are powerful tools for efficiently and accurately discriminating between Cuphea genotypes.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8 bit grayscale) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-06-14T18:17:07Z (GMT) No. of bitstreams: 1KarmarkarVidyadharM2004.pdf: 1799211 bytes, checksum: fb27e44ae927f0b23eb08fa904f7ded8 (MD5)
  • description.provenance : Submitted by Sergio Trujillo (jstscanner@gmail.com) on 2012-06-12T00:07:24ZNo. of bitstreams: 1KarmarkarVidyadharM2004.pdf: 826620 bytes, checksum: 5fbf2ed73c3b71de970e9db4c6b88767 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-06-14T19:01:25Z (GMT) No. of bitstreams: 1KarmarkarVidyadharM2004.pdf: 1799211 bytes, checksum: fb27e44ae927f0b23eb08fa904f7ded8 (MD5)
  • description.provenance : Submitted by Sergio Trujillo (jstscanner@gmail.com) on 2012-06-14T17:53:00ZNo. of bitstreams: 1KarmarkarVidyadharM2004.pdf: 1799211 bytes, checksum: fb27e44ae927f0b23eb08fa904f7ded8 (MD5)
  • description.provenance : Made available in DSpace on 2012-06-14T19:01:25Z (GMT). No. of bitstreams: 1KarmarkarVidyadharM2004.pdf: 1799211 bytes, checksum: fb27e44ae927f0b23eb08fa904f7ded8 (MD5) Previous issue date: 2003-08-05
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-06-13T17:31:46Z (GMT) No. of bitstreams: 1KarmarkarVidyadharM2004.pdf: 826620 bytes, checksum: 5fbf2ed73c3b71de970e9db4c6b88767 (MD5)
  • description.provenance : Rejected by Patricia Black(patricia.black@oregonstate.edu), reason: replace on 2012-06-14T17:39:47Z (GMT)

Relationships

Parents:

This work has no parents.

In Collection:

Items