Comparisons of chromomeric and puff patterns in the salivary gland and fat body chromosomes of Drosophila gibberosa third instar larvae and prepupae Public Deposited

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  • Chromosome maps were made of Drosophila gibberosa salivary gland and fat body chromosomes, and the puffing patterns were compared in the two tissues for selected times in development. The times were early third instar (E), late third instar (0, at pupariation (0 hr), at two hours (2 hr), four hours (4 hr), and ten hours (10 hr) after pupariation, and at pupation (18 hr). A comparison of the chromomeric patterns between the salivary gland and fat body tissues revealed one definite difference in banding pattern between the two tissues. Four bands were found in the fat body chromosome 2 at a site corresponding to a constriction in the salivary gland chromosome 2. Implications concerning the determination of the number of genes per band were discussed. A comparison of chromosomal puffing patterns between salivary gland and fat body tissues revealed more differences than similarities between the two tissues. More puffs with variable developmental patterns and fewer constant puffs were found in the salivary gland than in the fat body, suggesting that during development the salivary gland tends to vary its functions while the fat body tends to perform the same functions. Tissue specific puffs were found for both tissues and probably represent functions unique to a tissue. Puffing activity in the fat body was compared between feeding larvae (L) and metamorphosing prepupae (10 hr). Most chromosome regions showed the same activity pattern suggesting mostly the same functions were performed at both times. That a few regions differed in activity suggests there are processes found in the feeding larvae which are not found in the metamorphosing prepupae and vice versa. Generalized puffing occurs in the X chromosomes in both tissues. It is more extreme in the male, and may be attributable to dosage compensation. Puffing events in both tissues are discussed in relationship to what is known of ecdosyne as an effector of puffing sequence. The general types of developmental puffing patterns found in D. gibberosa are the same as those reported for D. hydei and D. melanogaster. For the larval and puparium formation sample times, the salivary gland and fat body chromosomes in D. gibberosa had fewer constant puffs in common than did the salivary gland, malpighian tubules and intestine in D. hydei (Berendes, 1966). Whether or not this means a greater diversity of function actually exists between the salivary gland and fat body tissues in D. gibberosa than exists among the salivary gland, malphigian tubules and intestine in D. hydei is questionable as the differences could be due to differences in experimental methods.
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