Graduate Thesis Or Dissertation

 

Aerobic biotransformation of chlorinated aliphatic hydrocarbons by a benzyl alcohol grown mixed culture : cometabolism, mechanisms, kinetics and modeling Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/qn59q652d

Descriptions

Attribute NameValues
Creator
Abstract
  • The aerobic transformation of TCE and cis-DCE by a tetrabutoxysilane-grown microorganism (Vancheeswaran et al., 1999) led to the investigation of novel substrates, including benzyl alcohol, for promoting cometabolism. The culture grew on carboxylic compounds and alcohols, but did not grow on formate, methanol, methane, propane, butane, ethylene, benzene, toluene, or p-xylene. Cis-DCE transformation was observed when the culture grew on butyrate, glucose, 1-propanol, 1-butanol, ethanol, benzyl alcohol, and phenol, and effectively transformed TCE, cis-DCE, and vinyl chloride when grown on phenol or benzyl alcohol. Several cycles of growth on benzyl alcohol led to increases in TCE transformation rates and transformation capacities. Products of benzyl alcohol degradation shifted from benzaldehyde to 2-hydroxy benzyl alcohol (2HBA) during the several cycles of growth. In resting cells studies, 2HBA production rates were highly correlated with TCE transformation rates. TCE transformation and 2HBA production rates doubled when the culture was grown on phenol and rates of TCE transformation were correlated with 2HBA production rates. Benzyl alcohol- and phenol-grown cells oxidized toluene to o-cresol, which indicated the similarity between benzyl alcohol ortho-monooxygenase, phenol hydroxylase, and toluene ortho-monooxygenase. 2-Butyne and 1-hexyne (but not acetylene) inhibited benzyl alcohol- and phenol-grown cells similarly, indicating the same ortho-monooxygenase was responsible for TCE cometabolism. Resting cell kinetic studies were performed with cells grown on phenol or benzyl alcohol. Benzyl alcohol degradation followed a Monod kinetics while phenol degradation followed a Haldane kinetics. The maximum transformation rates (k[subscript max]) of TCE, cis-DCE, and VC achieved by phenol-grown cells were about a factor of two higher than achieved with benzyl alcohol-grown cells, while the half-saturation constants (K[subscript s]) were in a similar range. Transformation capacities (Tc) for TCE, cis-DCE, and VC were about a factor of two to four higher with phenol-grown cells. The modeling of TCE, cis-DCE, and VC transformation using independently measured k[subscript max] and K[subscript s] values matched well with observed data from batch tests. Benzyl alcohol was shown to be an effective novel substrate for the aerobic cometabolism of TCE, cis-DCE, and vinyl chloride. Being a non-regulated compound, it might have applications for in-situ bioremediation.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2012-07-03T16:33:52Z (GMT). No. of bitstreams: 1 TejasenSarun2004.pdf: 1517314 bytes, checksum: 8b059138d1e7a078d7bb3d3b506849ca (MD5) Previous issue date: 2003-06-27
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-07-03T16:33:52Z (GMT) No. of bitstreams: 1 TejasenSarun2004.pdf: 1517314 bytes, checksum: 8b059138d1e7a078d7bb3d3b506849ca (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-07-03T16:26:06Z (GMT) No. of bitstreams: 1 TejasenSarun2004.pdf: 1517314 bytes, checksum: 8b059138d1e7a078d7bb3d3b506849ca (MD5)
  • description.provenance : Submitted by Kevin Martin (martikev@onid.orst.edu) on 2012-06-20T22:56:24Z No. of bitstreams: 1 TejasenSarun2004.pdf: 1517314 bytes, checksum: 8b059138d1e7a078d7bb3d3b506849ca (MD5)

Relationships

Parents:

This work has no parents.

Items