Graduate Thesis Or Dissertation
 

Glucose uptake and catabolism in the marine psychrophilic bacterium, Vibrio marinus

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  • The effects of various physical parameters on the binding, uptake, and catabolism of glucose in the marine psychrophilic bacterium, Vibrio marinus, were studied. It was shown that shortly after the cell was exposed to labeled glucose, the radioactive label became rapidly associated with the cell fraction. It was also shown that much of this activity was readily removed when the cells were exposed to an acidic environment. Since this release was not associated with leakage and the release was practically instantaneous, it appeared that this loss was related to the removal of loosely bound glucose. In order to reduce the chance of error in measuring substrate uptake, this loosely bound glucose was routinely removed by acidification prior to cell activity assay. The requirements for specific ions in the uptake of glucose were studied. It was found that Na⁺, Li⁺, and Mg⁺⁺ would permit uptake but that K⁺, Rb⁺, and NH₄⁺ would not. Of the various different salts of Na⁺ studied, all permitted glucose uptake. This was the same pattern that was seen when the effects of specific ions on cell growth were studied. The salinity limits of growth were defined, and their relationship to patterns of glucose uptake and respiration was observed. It was shown that there were no significant pattern shifts associated with the maximum growth salinity but that there were significant changes associated with the region of minimum growth salinity. When the effects of altering salinity on the uptake and respiration of specifically labeled glucose were studied, several patterns of potential, significance were observed. The total uptake of glucose, as measured by the first, second, third and sixth carbon activities, showed that all portions of the molecule were used for energy and cell material at 0,15 M NaCl but at higher salinities, part of the molecule was released by the cells in some form other than CO₂. Three basic patterns associated with the first and sixth carbons were observed as the salinity was increased from 0.0 M to 1.0 M NaCl. In the 0.0 M to 0.15 M range, the amount of CO₂ released from both carbons increased at approximately the same rate. In the 0.15 M to 0.30 M range, there was a decrease in the CO₂ associated with both of these carbons and a concomitant increase in the amount of cell activity associated with both of these carbons. As the salinity was increased above this point, the CO₂ associated with the sixth carbon remained relatively constant while that associated with the first carbon increased markedly. This shift in the C₆/C₁ ratio was shown to be relatively unaffected by changes in temperature over a wide range.
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