Effect of nutrients on interactions between two decay fungi and Trichoderma harzianum Rifai on ponderosa pine sapwood wafers Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/qn59q787q

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Small size wood assays were utilized to study the effect of added nutrients to the wood on the interactions of Trichoderma harzianum Rifai and two wood decay fungi, Trametes versicolor (white rot) and Gloeophyllum trabeum (brown rot). Weight losses related to time of exposure were monitored as a measure of decay. Total protein content from extracts obtained from ponderosa pine sapwood wafers treated with different levels of carbon or nitrogen compounds served as an indicator of biomass. The activities of selected wood degrading enzymes were studied in pure and mixed cultures of T. harzianum and the two decay fungi growing on the wood wafers. Weight losses for mixed cultures of T. harzianum and decay fungi indicated that the bioprotectant can delay the decay process. The results also suggested that T. harzianum was more active against brown rot fungi than the white rot fungi although completely inhibition did not occur with either decay fungus. The addition of carbon or nitrogen to the wood had a positive effect on T. harzianum performance against the two decay fungi studied but these nutrients also generally enhanced the growth of both decay fungi. The exception was glucose which had a negative effect on cellulase activity of Trametes versicolor. Trichoderma harzianum showed low hemicellulase and cellulase activity which is a desirable feature in a bioprotectant against wood decay but it stimulated laccase activity in the white rot Trametes versicolor. The reasons for this behavior are unknown and merit further study. The overall effect of Trichoderma harzianum on the decay fungi studied was reflected in decreased weight loss, total protein content and enzymatic activity except for laccase activity which was stimulated. Addition of sources of carbon or nitrogen to the wood enhanced the effect in most cases. The results indicate that the use of nutrients to enhance bioprotectant capabilities has potential although care must be taken to ensure that the nutrient do not stimulate the target organism.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Digital Production(digitalproduc@gmail.com) on 2010-09-14T19:19:26Z (GMT) No. of bitstreams: 1 RedactedCanessaEdwin1996.pdf: 4247428 bytes, checksum: cc8b533ba88b8d32bcbb88d69c98f2fd (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2010-09-17T21:19:09Z (GMT) No. of bitstreams: 1 RedactedCanessaEdwin1996.pdf: 4247428 bytes, checksum: cc8b533ba88b8d32bcbb88d69c98f2fd (MD5)
  • description.provenance : Made available in DSpace on 2010-09-17T21:19:09Z (GMT). No. of bitstreams: 1 RedactedCanessaEdwin1996.pdf: 4247428 bytes, checksum: cc8b533ba88b8d32bcbb88d69c98f2fd (MD5)
  • description.provenance : Submitted by Eric Vanderwall (ewscanner@gmail.com) on 2010-09-13T19:39:48Z No. of bitstreams: 1 RedactedCanessaEdwin1996.pdf: 4247428 bytes, checksum: cc8b533ba88b8d32bcbb88d69c98f2fd (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items