Graduate Thesis Or Dissertation
 

Aerobic degradation of chlorinated ethenes by Mycobacterium strain JS60 in the presence of organic acids

Pubblico Deposited

Contenuto scaricabile

Scarica il pdf
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/rj430750b

Descriptions

Attribute NameValues
Creator
Abstract
  • This study evaluated the potential of the aerobic Mycobacterium strain JS6O to grow on a variety of organic acid substrates, and the possible effects an organic acid would have on the degradation rate of vinyl chloride (VC). A series of batch growth tests were designed to determine the time it took to consume the substrate and the overall increase in biomass. Strain JS6O was found capable of growth on acetate, propionate, and butyrate, but could not grow on formate or lactate. Acetate was chosen for further study because strain JS6O consumed acetate the most rapidly of all the organic acids tested, and acetate is a common product of fermentation reactions in the subsurface. Strain JS6O was confirmed to grow on both ethylene and vinyl chloride as the sole carbon and energy source. Comparatively, strain JS6O's rate of growth on VC is much slower than that of ethylene. With acetate as an augmenting growth substrate, ethylene and VC utilization rates increased by 30% and 48%, respectively. Since acetate and VC are often found together in contaminated chlorinated ethene plumes, this makes a strong case for natural attenuation of VC by strain JS6O. A series of kinetic tests were implemented to determine the K[subscript s] and k[subscript max] of strain JS6O for ethylene, VC, and c-DCE. The K[subscript s] and k[subscript max] for ethylene determined through NLSR methods was similar to the values published in Coleman et al. (2002), supporting the maintenance of a pure culture throughout the experimental work. When strain JS6O was exposed to the isomers of DCE (trans-1,2-dichloroethylene (t-DCE), cis-1,2-dichloroethylene (c-DCE), and 1,1-dichloroethylene (1,1-DCE)) the cells were unable to grow on these compounds. However, when growing on acetate, strain JS6O cometabolized c-DCE and t-DCE, but not 1,1-DCE, with c-DCE transformed more rapidly than t-DCE. Transformation of c-DCE was also observed with growth on VC and ethylene. The presence of c-DCE was shown to partially inhibit VC degradation, but had no effect on ethylene degradation. The cometabolism results with acetate further indicate that strain JS6O is a good candidate for natural attenuation of multiple chlorinated ethenes in the subsurface.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Dichiarazione dei diritti
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces

Le relazioni

Parents:

This work has no parents.

In Collection:

Elementi

Thumbnail Titolo Data caricata Visibilità Azioni
file details BlatchfordChristina2006.pdf 2017-08-15 Pubblico Scaricare