Sterol uptake in Saccharomyces cerevisiae Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/rj430805q

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  • An assay is described to measure sterol uptake in Saccharomyces cerevisiae. This assay involves specific binding of radioactively labelled sterol to actively growing yeast cells. It was found that uptake does not occur in stationary cultures and that some growth is necessary for FY3 , a sterol auxotroph, to take up exogenous sterol. Uptake is maximized at pH 5.5 and in the absence of Tween 80 in short term (4 hour) assays. The optimal temperature for uptake was found to be about 31° C. Most uptake studies were carried out at 28° C for convenience. The sterol uptake system exhibits Michaelis-Menten kinetics and shows a Km of 62.9 μg/ml cholesterol under the conditions described. The activation energy is 94000 cal/mole.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-08-13T15:02:15Z (GMT) No. of bitstreams: 1 SalernoLaurelF1983.pdf: 222688 bytes, checksum: 9ad5ae113cf6063728850b247a785fad (MD5)
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  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-08-13T21:56:32Z (GMT) No. of bitstreams: 1 SalernoLaurelF1983.pdf: 222688 bytes, checksum: 9ad5ae113cf6063728850b247a785fad (MD5)
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