Determination of food carbon assimilation in copepods Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/rr172140v

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  • A method for measuring food carbon assimilation in the copepod Calanus finmarchicus was developed, using the diatom Ditylum brightwelli as food. The amount of particulate food carbon ingested was determined as the difference between CHN analyzer analyses for particulate food carbon of pre- and post-grazed food suspensions. The fraction of ingested food that was assimilated as organic matter was determined by a modified Conover gravimetric ratio method. This fraction was computed as: U=(F'-E')/(l-E')F' where F' and E' were the ash free dry weight:dry weight ratios of the copepods' food and feces, respectively. The fraction of ingested organic matter assimilated (U) multiplied by the amount of food carbon ingested estimated the amount of food carbon assimilated, assuming that the fraction of organic matter assimilated by the copepods was identical to the fraction of food carbon assimilated. Assimilation fraction determinations agreed well with those in the literature. The consistency in the carbon assimilation rates obtained apparently indicated a seasonal uniformity in absolute food carbon assimilated by adult Calanus.
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