A molecular study of the viral proteins of infectious hematopoietic necrosis virus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/rv042w50z

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  • The virion protein patterns of 72 isolates of infectious hematopoietic necrosis virus (IHNV) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of S-methionine labeled virus. This analysis led to the classification of these virus isolates into 4 or more types. Type 1 virus is characterized by a nucleocapsid protein with an approximate molecular weight of 40,500 daltons. The type 2 and 3 viruses have N proteins of 42,800 and 43,250 daltons respectively. Virus belonging to Type 2 was responsible for the recent epizootics of IHN among fish in the lower Columbia River. The California IHNV isolates were Type 3 with the exception of those isolated from fish at Coleman Hatchery on the Sacramento River. These Coleman Hatchery isolates belonged to a Type 4 virus group which are characterized by a larger G or glycoprotein of approximately 70,000 daltons. All other viruses examined have G proteins of 67,000 daltons. The Type 5 virus isolates were not sufficiently distinct to warrant classification into a separate type. These findings have been useful in determining that (1) a particular virus type is characteristic for a geographic area and will infect many different salmonid species in that area and (2) the same type isolated from parental fish is responsible for the subsequent outbreak of the disease in progeny. The specific radioactive labeling of virus proteins in the infected cell has been used to study the intracellular synthesis of viral proteins. The temporal synthesis of the viral polypeptides suggest that they were derived from the translation of independently transcribed monocistronic mRNAs. Antibody to IHNV was measured by solid phase direct binding assays with Iodinated Protein A or with immunoperoxidase staining. The high binding antibody titer of rabbit anti-IHNV serum made possible the development of two immunological tests for IHNV. Both methods were specific, sensitive to less than 10 ng of virus protein and represented a new method of characterizing different strains of IHNV.
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