Methionine biosynthesis in Saccharomyces cerevisiae : the one-carbon transfer portion of the pathway Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/rv042w52h

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  • The generation of the methyl group of methionine, and is also involved in thymidylic acid and purine biosynthesis. The understanding of the mechanisms and controls involved in one-carbon metabolism is incomplete, and is complicated by the existence of conjugated folate cofactors containing up to seven glutamic acid residues. The information that is available about the one-carbon transfer has been obtained using mainly the monoglutamyl tetrahydrofolate forms. Since the physiological balance of the conjugated derivatives could be critical, we preferred to utilize boiled cell extracts as a source of the cofactors. In this investigation, we have examined the one-carbon transfer in the unicellular eucaryote Saccharomyces cerevisiae. The generation of the methyl group of methionine (requiring the involvement of a polyglutamyl cofactor) was analyzed, and its relationship to thymidylic acid biosynthesis was explored. It appears that thymidylate synthetase may compete for polyglutamyl cofactors, and that the cellular dUMP concentration could influence methionine biosynthesis. It was also established that an interaction between S-adenosylmethionine and pyridoxal phosphate which can inhibit serine transhydroxymethylase, may cause growth inhibition of yeast when the cells are cultured in excess methionine.
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