Graduate Thesis Or Dissertation
 

Adaptation of molecular filtration for recovery of fish viruses from water

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  • The effectiveness of tangential flow filtration (TFF) for recovery of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) from large volumes of water was evaluated. In laboratory studies, virus recovery from IHNV-seeded water following concentration by TFF was approximately 13%. However, addition of 0.1 and 1% FBS to deionized water stabilized the virus, increasing virus recoveries to 95%. Addition of 0.03 and 0.3% beef extract resulted in IHNV recoveries of 80 and 61%, respectively. Similar results were obtained using IPNV-seeded water. Field studies using the TFF procedure were conducted from March to May, 1985, at a fish hatchery where IHNV is endemic. Infectious hematopoietic necrosis virus was detected in effluent from the adult steelhead trout (Salmo gairdneri) holding pond at an estimated concentration of one plaque-forming unit per five milliliters (1 pfu/5 ml). It was also detected at levels of 1 pfu/50 ml in water from a 2 m circular tank containing IHNV-infected steelhead trout fry. No virus was detected in the hatchery water supply.
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