Glutamine synthetase and α-Amylase activity in germinating wheat under water stress Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/s1784q958

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  • An integrated study of growth, enzyme activity, and water potential of wheat seeds germinated under mild water stress conditions was conducted to observe the relationship among the three kinds of parameters studied. From the relationship, the cause(s) or mechanism(s) of water stress in plant growth may be enlightened. Two important Pacific Northwest commercial cultivars, Hyslop and Wanser were studied under three mild stress conditions which often prevail at planting time in dryland areas of the Pacific Northwest. The stress conditions were simulated by mannitol solutions to provide -3, -6, and -9 bars of water potential. Seeds were germinated at 20-25°C in the dark and samples were taken daily. For seedling axis, water content, fresh and dry weights, soluble protein content, glutamine synthetase (GS) activity and total water potential were determined. For endosperm, a-amylase activity and total water potential were measured. Experimental results reveal that water potential, water content, fresh weight, dry weight, and soluble protein content of the seedling axis were progressively decreased with increasing stress. GS activity was reduced by the stress during the first two days of germination, then adaptation processes occurred resulting in comparable GS activity in all treated materials. The adaptive mechanism apparently occurs at the transcriptional level in producing more efficient GS in compensating the mild environmental stress. In endosperm, α-amylase activity was decreased by the stress treatments while water potential was increased by stress. As the treatments continued, a differential reduction expressed by the two cultivars occurred with the drought resistant Wanser being less affected or able to produce relatively more enzyme under stressed conditions. Based on the close relationship of water potential and soluble protein content in seedling axis, protein synthesis probably is the most sensitive system in plants to be affected by water stress. Under continuous mild stresses adaptive mechanism and genetic ability of adjustment seems to take effect and allow plants to cope with adverse conditions.
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