Purification and characterization of avian encephalomyelitis virus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/s4655j84k

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  • Avian encephalomyelitis virus-infected chick embryo homogenate was precipitated with polyethyleneglycol 6000 (PEG), extracted with fluorocarbon, concentrated by ultrafiltration, and purified by isopycnic gradient centrifugation. The physical and chemical properties of the purified virus were studied. The examination of purified avian encephalomyelitis virus (AEV) with electron microscopy using negative stain revealed virus particles which were icosahedral in shape, lacked an envelope, and had a mean diameter of 26 ± 3 nm. It was suggested that the viral capsid was composed of 12 capsomers located at the vertices of an icosahedron. The virus was tentatively given a triangulation number of one, and included in the icosahedral class P = 1. The sedimentation coefficient (S₂₀, w) of AEV was determined to be 148 ± 3 S. The bouyant density of the virus in CsC1 was determined to be 1.31 to 1.32 g/ml by infectivity assay following isopycnic gradient centrifugation. The infectivity titer of the virus was reduced by 1.2 log₁₀ after heating at 56 °C for one hour. The virus was stabilized to the effect of heat by 1 M MgCl₂ The virus was resistant to treatment with chloroform and trypsin. Attempts to discern the type of nucleic acid in the virus genome were inconclusive.
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