Graduate Thesis Or Dissertation

 

Purification and characterization of avian encephalomyelitis virus Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/s4655j84k

Descriptions

Attribute NameValues
Creator
Abstract
  • Avian encephalomyelitis virus-infected chick embryo homogenate was precipitated with polyethyleneglycol 6000 (PEG), extracted with fluorocarbon, concentrated by ultrafiltration, and purified by isopycnic gradient centrifugation. The physical and chemical properties of the purified virus were studied. The examination of purified avian encephalomyelitis virus (AEV) with electron microscopy using negative stain revealed virus particles which were icosahedral in shape, lacked an envelope, and had a mean diameter of 26 ± 3 nm. It was suggested that the viral capsid was composed of 12 capsomers located at the vertices of an icosahedron. The virus was tentatively given a triangulation number of one, and included in the icosahedral class P = 1. The sedimentation coefficient (S₂₀, w) of AEV was determined to be 148 ± 3 S. The bouyant density of the virus in CsC1 was determined to be 1.31 to 1.32 g/ml by infectivity assay following isopycnic gradient centrifugation. The infectivity titer of the virus was reduced by 1.2 log₁₀ after heating at 56 °C for one hour. The virus was stabilized to the effect of heat by 1 M MgCl₂ The virus was resistant to treatment with chloroform and trypsin. Attempts to discern the type of nucleic acid in the virus genome were inconclusive.
Resource Type
Date Available
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Georgeann Booth (gbscannerosu@gmail.com) on 2013-10-17T00:18:12Z No. of bitstreams: 1 GostingLarryH.pdf: 1803313 bytes, checksum: ba01f504dea50839f38f94248d2bca55 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-10-17T16:27:52Z (GMT) No. of bitstreams: 1 GostingLarryH.pdf: 1803313 bytes, checksum: ba01f504dea50839f38f94248d2bca55 (MD5)
  • description.provenance : Made available in DSpace on 2013-10-17T19:11:49Z (GMT). No. of bitstreams: 1 GostingLarryH.pdf: 1803313 bytes, checksum: ba01f504dea50839f38f94248d2bca55 (MD5) Previous issue date: 1977-06-15
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-10-17T19:11:49Z (GMT) No. of bitstreams: 1 GostingLarryH.pdf: 1803313 bytes, checksum: ba01f504dea50839f38f94248d2bca55 (MD5)

Relationships

Parents:

This work has no parents.

In Collection:

Items