Serological detection of staphylococcal enterotoxin A in culture supernatants Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/sj139475v

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  • A method has been presented for the observation of a large number of enterotoxin A producers. Production of a relatively concentrated toxin is achieved by the sac culture method. Further concentration, if needed, is carried out by PM-10 membrane ultrafiltration after centrifugation. The work is further improved by trypsinization of the supernatant fluid with 0. 08% trypsin to digest the interfering proteins and increase the percentage recovery during PM-10 membrane ultrafiltration. Detection is carried out by Laurell electroimmunodiffusion of the supernatant fluids. The mobility of the toxin on the antibody containing gel is increased as a result of trypsin treatment. The usefulness of the methods for the detection of small quantities of enterotoxin A in culture supernatants was investigated. Low producers of quantities of enterotoxin usually found in implicated foods (example S. aureus FRI #888 producing about 2.5 μg/ mll and L-19 producing 0.15 μg /ml) could be detected.
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  • description.provenance : Submitted by Georgeann Booth (gbscannerosu@gmail.com) on 2013-11-05T01:45:03Z No. of bitstreams: 1 NkangaEnefiok1976.pdf: 339876 bytes, checksum: cf73426e8c13173ee24aa245c6cc5c0b (MD5)
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-11-05T16:38:40Z (GMT) No. of bitstreams: 1 NkangaEnefiok1976.pdf: 339876 bytes, checksum: cf73426e8c13173ee24aa245c6cc5c0b (MD5)
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