Graduate Thesis Or Dissertation
 

The cysteine to homocysteine conversion in Saccharomyces cerevisiae

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/sn00b1974

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  • The mechanism of transsulfuration and the role of cystathionine in the biosynthesis of methionine in yeast were investigated. Saccharomyces were shown to accumulate cystathionine by use of the ³⁵S labeled compound. Cell-free extracts of the wildtype clone as well as methionine auxotrophs were shown to cleave cystathionine. The enzymatically produced compounds were shown to be pyruvic acid and homocysteine. Pyruvic acid was determined by the Rf value of the 2, 4-dinitrophenylhydrazone as compared to authentic. The absorption spectrum of the phenylhydrazone of authentic pyruvate was identical to that of the phenylhydrazone of the enzymatic reaction mixture. The sulfhydryl was shown to be homocysteine by comparing the Rf values of the radioactive cleavage product to a ninhydrin and sulfur positive reference spot of homocysteine, and by bioautographic procedures. Transsulfuration and the role of cystathionine in methionine biosynthesis in yeast were discussed in view of these results which varied somewhat from that which has been found for other organisms.
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