Graduate Thesis Or Dissertation
 

Nitrogen use in container-grown woody ornamentals

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/sq87bx680

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  • Lasting, effective solutions to nitrogen (N) loss from container nurseries must address layers of complexity ranging in scale from whole system nursery management to gene expression. Group-based On-site Active Learning (GOAL) was developed to aid nursery managers and related stakeholders (e.g. neighbors, policy makers, regulating agencies, researchers) in developing a better understanding of how nitrogen flows through container nurseries and the effects of N management decisions over space and time. After completing GOAL, 94% of participants indicated that they learned a new idea or concept about N cycling in their container nursery. New ideas and concepts from peers and colleagues were gained by 100% of participants, 60% from researchers, and 60% developed their own ideas and concepts. Controlled release fertilizers (CRFs), applied to reduce N losses, introduce substantial amounts of N to production systems, and their release patterns often do not match plant requirements. Commercially comparable plants were grown under a precision liquid fertilization regime with 68.7% (Euonymus alatus 'Compactus', slow growing) and 48.6% {Weigelaflorida 'Red Prince', fast growing) less N than was introduced to the production system with CRF treatments yielding the highest dry weights and total plant N. Various rates (25, 50 100, 200, and 300 mg-L⁻¹) of ¹⁵N depleted NH₄NO₃ (min 99.95% atom ¹⁴N) were applied to three container-grown woody ornamentals. Estimation of N recovery determined by total N in the plant was significantly higher than estimation of N recovery determined by labeled fertilizer N in the plant at low N rates. Increasing fertilizer rates up to 100 mg-L"1 resulted in increased uptake of nitrogen derived from other sources (NDFO), and NDFO at low N concentrations was a significant portion of the total N in the plant. As a result, the nonisotopic total N method overestimates fertilizer N uptake three to four times in container-grown plants at N concentrations of 25 mg-L⁻¹. A pair of degenerate primers were developed that consistently amplify a ~635 nucleotide section of the NRTl gene (nitrate transport) in Rhododendron 'Unique' and Cornus sericea. PCR products were cloned and sequenced and 79.52 % of nucleotides matched between Rhododendron 'Unique' and Cornus sericea.
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file details SANDROCKDAVIDR2004.pdf 2017-08-19 Pubblico Scaricare