Graduate Thesis Or Dissertation
 

Influence of atmospheric humidity and free water on germination and germ tube growth of Botrytis cinerea Pers.

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  • The influence of atmospheric humidity and free water on germination and germ tube growth of Botrytis cinerea Pers. ex Fr. was studied. Relative humidities (RH) were maintained inside insulated humidity chambers with sulfuric acid solutions of the appropriate concentrations. Insulation was necessary to reduce the incidence of condensation caused by temperature fluctuations. Any condensation occurring during incubation at humidities above 90% RH was detected with ink dust on filter paper. Conidia were dusted on dialysis membranes and placed at humidities of 70, 90, 94, and 98% RH. Samples removed after 12, 24, 36, and 60 hr were compared to controls that had been placed in distilled water (dH₂O). Conidia germinated in the control but not at any RH tested. Further studies were conducted to determine whether or not free water was necessary throughout the germination process. Conidia were dusted on dialysis membranes and incubated in dH₂O for 1, 2, and 3 hr. Samples were removed after each interval and percent germinati6n was measured. Conidia were then incubated at 98% RH. Samples removed after 12, 24, 36, and 60 hr were compared to initial samples. Percent germination did not increase after incubation at 98% RH. The influence of atmospheric humidity on germ tube growth was investigated. Conidia were dusted on dialysis membranes and placed in dH₂O for 4.5, 8, and 12 hr to promote germination and germ tube growth. Samples were removed after each interval, and germ tube lengths were measured. Germinated Conidia were then exposed to humidities of 70, 90, 94, and 98% RH. Germ tube lengths of samples removed from humidity chambers after 12, 24, 36, and 60 hr intervals were compared to the 12 hr control that had remained in dH₂O. Germ tube lengths did not increase when incubated at any RH tested. Results suggest that water films on plant surfaces are essential for germination and pre-penetration germ tube growth of B. cinerea.
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