The influence of selenium on selenoprotein W Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/t435gg38b

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  • The objective of our study was to investigate the effect of selenium on selenoprotein W. Western blot analysis indicated that selenoprotein W is present in muscle, brain, testis and spleen of rat tissues. Tissue distribution of selenoprotein W was not altered in rats fed various selenium levels. Among muscle, brain, testis and spleen, selenoprotein W in muscle was most responsive to selenium status, but brain appeared to be the least responsive organ. Northern blot data indicated that selenoprotein W mRNA in rat muscle increased significantly as levels of selenium supplementation increased. Western blot data indicate that selenoprotein W in rat muscle is non-detectable until selenium supplementation increased to 0.06 ppm, and the level of selenoprotein W in muscle reached a plateau at 1 ppm selenium supplementation where no further increase occurred. Except brain, selenoprotein W was higher in all tissues in sheep fed a selenium supplemented diet than in those fed the deficient diet. However, selenoprotein W levels were not different in brains between selenium-deficient and selenium-supplemented sheep. In contrast to rats, selenoprotein W was as high in the heart as in the muscle of selenium-supplemented sheep. In the study with L8 muscle cells, selenoprotein W did not change significantly during cell differentiation, indicating that selenoprotein W was not affected by muscle cell differentiation. Selenite was the most effective form of selenium for selenoprotein W synthesis in these muscle cells. Selenoprotein W level reached a plateau when L8 myotubes were incubated with 10⁻⁷ M selenium, whereas selenoprotein W mRNA reached a plateau with 10⁻⁸ M selenium.
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  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
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  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-11-05T18:16:42Z (GMT) No. of bitstreams: 1 Yeh JanYing1996.pdf: 9150579 bytes, checksum: 70bbfd774dbbfe5867fec239464687f0 (MD5)
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  • description.provenance : Made available in DSpace on 2012-11-05T21:48:04Z (GMT). No. of bitstreams: 1 Yeh JanYing1996.pdf: 9150579 bytes, checksum: 70bbfd774dbbfe5867fec239464687f0 (MD5) Previous issue date: 1995-11-20
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-11-05T21:48:04Z (GMT) No. of bitstreams: 1 Yeh JanYing1996.pdf: 9150579 bytes, checksum: 70bbfd774dbbfe5867fec239464687f0 (MD5)

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