Fertilization of salmonid eggs with cryopreserved sperm Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/t435gg75g

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  • Numerous fishery researchers have attempted to preserve viable fish sperm, but only three investigations were successful in the fertilization of fresh eggs with frozen and thawed sperm: Blaxter (1953); Hoyle and Idler (1968); and Graybill and Horton (1969). Significant advances reported in this thesis were: the development of suitable life extenders for semen of steelhead trout (Salmo gairdneri), and coho (Oncorhynchus kisutch) and Chinook (0. tshawytscha) salmon; the selection of a life protector composed of 8.0% dimethyl. sulfoxide; the verification that the best rate of freezing semen was -30 C/mm, arid that the best method of thawing frozen sperm was in a 4 C water bath; and the determination that no equilibration time was necessary when fresh semen was diluted with extenders and protectors. Application of the above techniques led to the successful cryo-preservation of male gametes of steelhead trout and Chinook and coho salmon in liquid nitrogen (-196 C). Frozen and thawed sperm was mixed with fresh eggs and resulted in maximum fertilizations of 59.1% for steelhead trout, 38.4% for spring Chinook salmon, and 79.2% for coho salmon. Details of the chemical constituents of the extenders and protectors tested are appended.
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  • File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
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  • description.provenance : Approved for entry into archive by Anna Opoien(anna.opoien@oregonstate.edu) on 2011-08-12T17:30:26Z (GMT) No. of bitstreams: 1 OttAlvinGeorge1970.pdf: 522207 bytes, checksum: d3bebaf841f7593aee0c1610d8250190 (MD5)
  • description.provenance : Submitted by Tamera Ontko (toscannerosu@gmail.com) on 2011-08-02T21:44:53Z No. of bitstreams: 1 OttAlvinGeorge1970.pdf: 522207 bytes, checksum: d3bebaf841f7593aee0c1610d8250190 (MD5)
  • description.provenance : Approved for entry into archive by Anna Opoien(anna.opoien@oregonstate.edu) on 2011-08-12T17:04:24Z (GMT) No. of bitstreams: 1 OttAlvinGeorge1970.pdf: 522207 bytes, checksum: d3bebaf841f7593aee0c1610d8250190 (MD5)
  • description.provenance : Made available in DSpace on 2011-08-12T17:30:26Z (GMT). No. of bitstreams: 1 OttAlvinGeorge1970.pdf: 522207 bytes, checksum: d3bebaf841f7593aee0c1610d8250190 (MD5) Previous issue date: 1969-09-04

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