Graduate Thesis Or Dissertation

 

Characterization of cytokinin metabolic enzymes from Phaseolus Public Deposited

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  • An enzyme catalyzing the formation of O-glucosylzeatin in immature embryos of P. lunatus was purified 2500 fold using ammonium sulfate precipitation followed by affinity and anion exchange chromatography. The enzyme uses trans-zeatin as substrate (Km 28 uM) but not cis-zeatin. ribosylzeatin, or dihydrozeatin. Both UDP-glucose and UDP-xylose can serve as glycosyl donors, with Kms of 0.2 and 2.7 mM respectively, for the formation of O-glucosylzeatin and O-xylosylzeatin. In comparison, the UDPxylose:zeatin O-xylosyltransferase (Turner et al., 1987) isolated by the same procedures from P. vulgaris embryos uses only UDP-xylose as donor substrate and the Kms for both zeatin and UDP-xylose are much lower (2 and 3 micromolar, respectively). The chromatographic behavior on affinity columns and the molecular weights (approximate Mr 44,000 daltons) of the two enzymes are similar. Results from substrate competition experiments and enzyme separation by anion exchange HPLC indicate a single, distinct, zeatin O-glycosylation enzyme occurs in embryos of each of these Phaseolus species.
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