An investigation into the humoral immune response of the ascidian urochordate, Styela montereyensis Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/t435gh45z

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Adaptive immune capabilities of the Urochordate S. montereyensis were studied. Animals repeatedly injected with bacteria were bled and the following properties were measured in vitro: agglutinin, bactericidin, bacteria-cell association. Both Gram (+) and Gram (-) bacteria were isolated from the gut of S. montereyensis and used as antigens at 10⁸ bacteria ml⁻¹, 0.01 ml g⁻¹. Tests conducted over 25 days, with injections and samples made every two and five days respectively, failed to reveal a natural or induced bactericidin. Though increases in phagocytic rates and agglutinin titer were small under the aforementioned protocol, the groups injected with bacteria all showed positive trends with time following initial declines when compared to the saline-injected group. It was not clear whether these trends reflected a return to the basal level (with positive feedback) or whether they constituted induced responses similar to the adaptive immune responses common in the vertebrates. The data do not indicate specificity with regards to the Gram (+) or Gram (-) antigen. The health of the test animals was monitored by weighing each animal at the start of the experiment and again when the animals were sampled. No apparent correlation existed between the health of the animal and the values measured in the agglutinin and bacteria-cell association assays. The uninjected controls gained weight and the saline-injected group lost weight, yet both had similar assay values. In contrast to the saline-injected group, the bacteria-injected groups showed increasing trends while losing weight. Laboratory specimens of S. montereyensis were maintained by supplying cultures of Pseudoisochrysis paradoxa. The immunological study was preceded by experiments to select a food on which S. montereyensis was capable of weight-gain under laboratory conditions. Of three possible foods, S. montereyensis gained weight only when fed quantities greater than 22,000 cells animal⁻¹ day⁻¹ of P. paradoxa. The gain in wet weight was shown to be related to tissue growth.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6670 in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Kirsten Clark(kcscannerosu@gmail.com) on 2013-10-25T18:48:24Z (GMT) No. of bitstreams: 1 DeWanDaleF1978.pdf: 1214632 bytes, checksum: a6c9ac1c1b9879bfa8450dcba3c94c94 (MD5)
  • description.provenance : Submitted by Katy Davis (kdscannerosu@gmail.com) on 2013-10-09T22:09:48Z No. of bitstreams: 1 DeWanDaleF1978.pdf: 1214632 bytes, checksum: a6c9ac1c1b9879bfa8450dcba3c94c94 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2013-10-15T17:03:44Z (GMT) No. of bitstreams: 1 DeWanDaleF1978.pdf: 1214632 bytes, checksum: a6c9ac1c1b9879bfa8450dcba3c94c94 (MD5)
  • description.provenance : Made available in DSpace on 2013-10-25T18:48:24Z (GMT). No. of bitstreams: 1 DeWanDaleF1978.pdf: 1214632 bytes, checksum: a6c9ac1c1b9879bfa8450dcba3c94c94 (MD5) Previous issue date: 1978-02-23

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items