The effect of DDT on infectivity of RNA tumor viruses Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/t722hd260

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  • Chicken embryonic cells (CEC) cultured in vitro were able to withstand the toxicity of DDT at concentrations below 10μg/ml culture medium. When the DDT concentration exceeded the toxic level, CEC appeared vacuolated and lost the spindle-like fibroblastic appearance, Gas chromatographic analysis had shown that the uptake of DDT (3μg/ ml'culture medium) by about 10⁷ CEC was 0. 93 μg in 72 hours. At this DDT concentration, the infectivity of the RNA tumor virus MC29 in CEC was inhibited by approximately 80% in 72 hours when DDT was added to the cultures immediately after infection. Short terms of pretreatment, as low as two hours, before infection inhibited virus infectivity nearly as much as continuous DDT treatment after infection. CEC received only six hours of DDT pretreatment before infection was found to be more susceptible to MC29 infection than CEC that received the six hour pretreatment before infection and continuous treatment after infection. Susceptibility of virus infection was measured by cell transformation in terms of focus forming units. A virus-specified protein (reverse transcriptase enzyme) was reduced by about 50% in 72 hours under continuous DDT treatment. The enzyme from DDT treated cultures sedirnented at about the characteristic sedimentation coefficient (8. 2S). DNA synthesis of pretreated cells measured by ³H-thymidine incorporation changed little. MC29 virus adsorption (60-70%) was found unaffected by DDT.
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