Graduate Thesis Or Dissertation
 

Cell division and DNA synthesis in Saccharomyces cerevisiae following nutritional shiftup

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  • Nutritional shift-up experiments have revealed many important macromolecular interactions in bacteria. It has been shown that RNA synthesis can be dissociated from protein and DNA synthesis. The rates of protein synthesis were found to be, at a given temperature, strictly dependent on the numbers of mature ribosomes present. DNA synthesis remained unaffected by the increased rates of RNA and protein synthesis for some time after shift-up. The rate of cell division was not coupled to the new rates of RNA, DNA, and protein synthesis for one pre-shift generation time after the shift. Recent experiments with yeast, however, have indicated that the rates of DNA synthesis and cell division are tightly coupled to the rates of RNA and protein synthesis after nutritional shift-up. Our experiments have demonstrated that these results are in error. The rates of cell division are maintained in yeast for one generation time after shift. Also a rate maintenance phenomenon is observed with respect to DNA synthesis. These results indicate that the times of genome replication and the time for cell division to occur are constant between generation times of 120 300 minutes. Under our experimental conditions mitochondrial DNA is preferentially synthesized during the first 20- 30 minutes after shift-up. There is some indication that this preferential synthesis is due to partial respiratory adaptation.
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