Graduate Thesis Or Dissertation
 

Interrelationships among changes in flavor and aroma, and composition of stored strawberry juice concentrate

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  • Sensory evaluation and instrumental methods were applied to the evaluation of strawberry juice concentrate (68°Brix) stored at 20°C which had been produced both commercially (C-SJC) and in a pilot plant (SJC). Sensory evaluation included taste and aroma ratings by intensity scaling and time-intensity of taste, and visual colorimetry by matching Munsell color chips. Instrumental analyses included tristimulus colorimetry (i.e. Hunter colorimeter), spectrophotometric colorimetry for pigment analyses, titrametric analyses for acidity (pH and titratable acidity) and free α-amino acids (formol number), and headspace gas analyses for CO₂ and O₂. During six days storage of C-SJC, a decrease in concentration of anthocyanins and increase in polyphenolics (tannin) was associated with an increase in astringency. Free α-amino acids were observed to decrease, while CO₂ was released. These changes were associated with an increase in musty/moldy and pungent aromas. Free sugars and titratable acidity did not change. The pilot plant SJC was processed from blanched and unblanched fruit to evaluate the relative importance of oxidase activity (i.e. polyphenoloxidase) prior to pasteurization. The blanching treatment increased the astringency and sourness in unstored SJC. These affects were associated with an increase in concentration of polyphenolics (tannin). During storage, the blanch treatment decreased the rate of anthocyanin loss and decreased the release rate of CO₂, yet degradation rates were still high. The 0₂ concentration in headspace did not change significantly during storage indicating that polyphenoloxidase (PPO) activity during storage was low. The musty/moldy and pungent aromas increased similarly to C-SJC. A chemical mechanism accounting for these changes is proposed where products from the oxidative degradation of ascorbic acid contribute directly or indirectly to the degradation of anthocyanins to yield browning. Further, high initial concentrations and subsequent decreases during C-SJC storage of free α-amino acids indicate that Strecker degradation is a participating mechanism. Associations of browning with the development of off-flavors suggest this chemical mechanism forms odor-active volatile compounds.
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