Graduate Thesis Or Dissertation

Effect of dietary fat and cholesterol on plasma lecithin:cholesterol acyltransferase activity in man

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  • Effect of diets varying in amount of cholesterol and polyunsaturated to saturated fatty acid ratio (P/S) on plasma lipid levels and lecithin:cholesterol acyltransferase (LCAT) activity was determined in fourteen young adult male subjects during a 13-week experimental period in a crossover design. Control diet was fed for two weeks prior to 11-week experimental period. Two experimental diets were sequentially fed to each group during the 11-week experimental period switching the diet at the midpoint. The blood was drawn five times and LCAT activity and plasma lipid levels were measured. The effect of fat modifications in the diets on fractional LCAT activity showed a systematic change in LCAT activity independent of diet. Plasma total cholesterol levels were significantly (p < 0.05) elevated by diets containing high saturated fat with either high (22%) or low (7%) cholesterol while diets high in polyunsaturated fat with either high or low cholesterol levels lowered the total cholesterol in Groups I and II but increased it in Groups III and IV. Trend of changes in plasma triglyceride was similar to that of plasma LCAT in Groups I and IV. However, there was inconsistency in the relationship between the LCAT activity and plasma lipid levels. LCAT enzyme was purified by 436-fold from plasma with 14% yield.
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