Graduate Thesis Or Dissertation
 

Synthesis of Glutamic-γ-semialdehyde in Escherichia coli : isolation of a low molecular weight compound required for in vitro synthesis

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  • Preliminary work on a crude in vitro system for glutamic-γ-semialdehyde synthesis in Escherichia coli has been described. The cell extract synthesis of GSA is inhibited by proline; other allosteric characteristics observed include sigmoidal substrate variation and inhibition plots of activity. The membrane fraction and a low molecular weight compound in the soluble fraction were components of the cell extract required for in vitro synthesis. Emphasis of this work was on the isolation and characterization of the low molecular weight compound. An isolation procedure is described by which relatively large amounts of this cofactor can be partially purified. Some characteristics of this compound have been determined, and a possible mode of action is described.
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