Graduate Thesis Or Dissertation
 

Development of a marine bioassay system using the gooseneck barnacle, Pollicipes polymerus

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/v692t859z

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  • The purpose of this study was to develop a marine bioassay procedure using eggs and larvae of Pollicipes polymerus, a stalked barnacle common on the west coast of North America. A series of experiments were run to determine optimal culture conditions for the eggs, to see if they could be grown without antibiotics, and to generate the necessary data on development time, hatching, and molting success required to design future experiments. Next, two bioassays were run to determine how the system performed in actual use. A phthalate ester plasticizer, dibutyl phthalate (DBP), was used as the test compound. The first of these experiments indicated there was a significant drop in molting success from larval stage I to stage II when eggs were exposed to 1000 ppb DBP. Variances in molting success, sometimes high in previous experiments, became unacceptable in this one, so the next experiment was designed to reduce these variances by more accurately identifying the time of first egg hatching. The end point used in previous experiments (3 days after first hatching) proved to be insufficient for enough of the controls to molt and, therefore, no conclusions could be drawn on the effects of DBP. The experiment did indicate how excessive variance in molting success could be avoided in future experiments, and how overall molting success could be improved. Hatching successes obtained in all these experiments compared favorably to those obtained by previous investigators. While testing the synthetic seawater used in the experiments for background phthalate levels, it was found that several phthalates, particularly DBP and diethylhexyl phthalate (DEHP) were persistent contaminants. Dibutyl phthalate levels could be reduced by treatment with activated charcoal. Tests were conducted to determine how much DBP remained in solution between water changes. Dibutyl phthalate loss was found to increase with increasing initial DBP concentration, but DBP loss from this system over 24 hours compared favorably to that observed in a similar experiment employing larval marine crustaceans. A design for equipment to culture P. polymerus in vitro and suggestions for conducting bioassay studies with eggs and larvae are described.
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