Bacterial 16S ribosomal DNA analysis of pyrrolizidine alkaloid detoxifying enrichments from the ovine rumen Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/v979v593s

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were identified using their 16S ribosomal RNA genes. Gene sequences from a rich medium enrichment were matched to Clostridium bifermentans, Prevotella ruminicola, Escherichia coif, and from a minimal medium enrichment to, C. clostridiiforme, C. aminophilum, Streptococcus bovis, and Butyrivibrio fibrosolvens. There were no identical organisms between the two libraries, but the common genus was Clostridium.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome, 256 Grayscale) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Submitted by Kirsten Clark (kcscannerosu@gmail.com) on 2012-10-08T17:34:13Z No. of bitstreams: 1 GrayDianeR1998.pdf: 3763664 bytes, checksum: 92d12e7357fe3fc847faaf15635b0db1 (MD5)
  • description.provenance : Made available in DSpace on 2012-10-08T18:39:05Z (GMT). No. of bitstreams: 1 GrayDianeR1998.pdf: 3763664 bytes, checksum: 92d12e7357fe3fc847faaf15635b0db1 (MD5) Previous issue date: 1998-02-05
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-10-08T18:39:05Z (GMT) No. of bitstreams: 1 GrayDianeR1998.pdf: 3763664 bytes, checksum: 92d12e7357fe3fc847faaf15635b0db1 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-10-08T18:37:22Z (GMT) No. of bitstreams: 1 GrayDianeR1998.pdf: 3763664 bytes, checksum: 92d12e7357fe3fc847faaf15635b0db1 (MD5)

Relationships

In Administrative Set:
Last modified: 08/15/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items