The early pathogenesis of Pasteurella multocida infection studied by immunohistochemical techniques Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/vd66w4950

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  • Initial sites of localization and multiplication of Pasteurellamultocida were examined in chickens intravenously inoculated withvarying doses of bacteria. Sections of lung
  • Initial sites of localization and multiplication of Pasteurellamultocida were examined in chickens intravenously inoculated withvarying doses of bacteria. Sections of lung, liver, kidney, bonemarrow and spleen were prepared for light microscopic examination.Tissue sections were stained with the hematoxylin and eosin stain,Gram's stain or the avidin-biotin-peroxidase complex immunoenzymatictechnique. Of the three, immunohistochemistry proved to be far moresensitive in localizing bacteria within the various organs. Asearly as five hours postinoculation, bacteria were evident in lownumbers in splenic and hepatic mononuclear phagocytes. Bacteriawere not observed within the other organs or the blood. Withincreasing time, bacteria were observed in large numbers withinthese cells, causing necrosis of phagocytes, and liberating bacteriainto the sinusoids. In birds succumbing to the infection, bacteriawere seen in large numbers in all organs and in the blood, bothintracellularly and extracellularly. These results are consistentwith the hypothesis that P. multocida multiplies within hepatic andsplenic phagocytes in an early phase of infection. The possibilitythat Pasteurella multocida may be able to resist intracellularkilling and may in fact multiply within the mononuclear phagocyte isdiscussed.
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