Graduate Thesis Or Dissertation

 

The early pathogenesis of Pasteurella multocida infection studied by immunohistochemical techniques Public Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/vd66w4950

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  • Initial sites of localization and multiplication of Pasteurellamultocida were examined in chickens intravenously inoculated with varying doses of bacteria. Sections of lung, liver, kidney, bone marrow and spleen were prepared for light microscopic examination.Tissue sections were stained with the hematoxylin and eosin stain,Gram's stain or the avidin-biotin-peroxidase complex immunoenzymatictechnique. Of the three, immunohistochemistry proved to be far more sensitive in localizing bacteria within the various organs. As early as five hours post inoculation, bacteria were evident in low numbers in splenic and hepatic mononuclear phagocytes. Bacteria were not observed within the other organs or the blood. With increasing time, bacteria were observed in large numbers within these cells, causing necrosis of phagocytes, and liberating bacteriainto the sinusoids. In birds succumbing to the infection, bacteria were seen in large numbers in all organs and in the blood, both intracellularly and extracellularly. These results are consistent with the hypothesis that P. multocida multiplies within hepatic and splenic phagocytes in an early phase of infection. The possibility that Pasteurella multocida may be able to resist intracellular killing and may in fact multiply within the mononuclear phagocyte is discussed.
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