Catabolic pathways of carbohydrate in the intermolt crayfish, Pasifastacus leniusculus Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/vh53wz14f

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  • The radiorespirometric method was used to study the catabolism of glucose in intermolt crayfish. Rapid and extensive conversion of C-3(4) of glucose to C¹⁴O₂ indicated that a significant portion of the administered glucose was routed into the glycolysis- Krebs cycle sequence. High yields of C-6 of glucose in the respiratory CO₂ suggested the participation of the glucuronate pathway. Some pentose phosphate pathway activity was suggested by the fact that C-1 of glucose was converted to CO₂ at a slightly higher rate than C-2 in the earlier portions of the time -course experiments. Further evidence for participation of the latter two pathways was obtained by administering glucuronate-6-C¹⁴ and gluconate-1-C¹⁴ Cumulative (70 hours) C¹⁴O₂ yields of 70 percent and 50 percent were obtained respectively, indicating extensive conversion of these substrates to respiratory CO₂.
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