Studies involving substrate binding, uptake, and respiration near the minimum growth temperature of Escherichia coli Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/w0892f30q

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Escherichia coli K12, UC41 (an auxotroph) and UC175 (a wild type) were used to determine possible relationships between binding, uptake, and respiration and minimum growth temperature. Both E. coli strains had a minimum growth temperature of 8.0 C in glucose minimal medium when cells were previously grown at 15 C. UC41 showed a greater uptake rate of the amino acids than did UC175. A nearly constant uptake rate of L-methionine by UC41 below the minimum growth temperature was seen regardless of the wash treatment used during filtration. At and above the minimum growth temperature, uptake increased with increasing temperature indicating a direct relationship between binding and uptake and the minimum growth temperature. Cells of UC41 which were osmotically shocked to remove the histidine-binding protein were unable to resynthesize new binding protein below 8 C. The auxotroph also had a smaller uptake constant for arginine at and above the minimum growth temperature than below.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2014-01-07T21:09:16Z (GMT). No. of bitstreams: 1 GoodrichThomasDale1974.pdf: 895932 bytes, checksum: ba940a4f5da6f1a15b81b3a23ee9436f (MD5) Previous issue date: 1973-08-01
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-01-07T21:09:16Z (GMT) No. of bitstreams: 1 GoodrichThomasDale1974.pdf: 895932 bytes, checksum: ba940a4f5da6f1a15b81b3a23ee9436f (MD5)
  • description.provenance : Submitted by Sergio Trujillo (jstscanner@gmail.com) on 2013-12-18T21:29:03Z No. of bitstreams: 1 GoodrichThomasDale1974.pdf: 895932 bytes, checksum: ba940a4f5da6f1a15b81b3a23ee9436f (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-01-06T17:57:21Z (GMT) No. of bitstreams: 1 GoodrichThomasDale1974.pdf: 895932 bytes, checksum: ba940a4f5da6f1a15b81b3a23ee9436f (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items