Differential agar media for the identification of the Salmonella and Arizona groups of Enterobacteriaceae Public Deposited

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  • Twelve differential agars have been studied in the development of media to presumptively identify the Salmonella Subgenera I-IV. Medium No. 7B/A (or Medium No. 7-H₂S Strip) combined the malonate, dulcitol, lysine decarboxylase and hydrogen sulfide reactions in one tube. It was designed to be used at the TSI stage of analysis when typical Salmonella colonies are found on selective plates, especially XLD. This medium was found effective in the differentiation of the typical Salmonella within 24 to 30 hours when tested with 51 known Enterobacteriaceae cultures. Medium No. 10 was designed for use with Bismuth Sulfite Agar plates when other selective plates give atypical or lactose fermenting reactions. It combined the sucrose, malonate and hydrogen sulfide reactions in one tube. Medium No. 10 was not found to be a suitable replacement for TSI, but gave valuable information when inoculated in parallel with TSI, as it ascertained the malonate reaction and confirmed the sucrose reaction. In the study of 85 cultures from 14 samples, two Arizona and one false positive (for Arizona) Citrobacter culture were found. The majority of isolates were malonate positive, dulcitol negative, sucrose negative, hydrogen sulfide negative and lysine decarboxylase negative. No false positives were found for Subgenera I and II. When incorporated into conventional procedures, Medium No. 7B/A should allow the presumptive identification of Salmonella subgenera 24 hours earlier than is now possible. Malonate Dulcitol Lysine Iron Agar (MDLI) is the name proposed for this medium.
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