Neutral lipid metabolism in the fat body of the larva of the fleshfly, Sarcophaga bullata Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/w3763914c

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • The purpose of this study was to characterize the neutral lipids of the fat body of the larva of the fleshfly Sarcophaga bullata and to examine the means by which these lipids are synthesized. The weight of the larva, the fat body as well as its lipid content were examined in relationship to the growth of the larva. The average larva attained maximum body weight of about 220 mg in 70 hours. However, fat body lipid continued to increase in weight after the feeding stage of the larva during which time there was a small decrease in total larval wet weight. The fat body lipid comprised mainly of the neutral lipid and triglyceride formed the major portion of the neutral lipid. Approximately 60% of the total fatty acids analysed was found to be unsaturated and an increase in unsaturated fatty acid content was found in the fat body of the nine day old larvae. The major portion of fatty acids in the fat body as analysed by gas-liquid chromatography were palmitic, palmitoleic, stearic, oleic and linoleic acids. Very small amount of acetate-1-C¹⁴ was incorporated into the fat body lipid. Palmitate-1-C¹⁴, on the other hand, was more extensively incorporated. Most of the radioactivity which was incorporated into the neutral lipid was present in the three glyceride fractions, of which the diglycerides showed the highest specific activity. It is suggested that diglyceride could play an important role in lipid transport in the insect system (13, 14). Incorporation of palmitate-1-C¹⁴ into fat body lipid during different time intervals of incubation indicated that there was a very rapid uptake of the radioactive material in the first 30 minutes. Comparison of palmitate-1-C¹⁴ incorporation between the three day and seven day old larvae showed that larvae at their prepupal stage were still able to incorporate long chain fatty acids but to a somewhat less extent. Fatty acids of neutral lipid were separated into saturated and unsaturated components on thin-layer plates. After incubation of fat body of three day old larvae with palmitate-1-C¹⁴, the ratio of the distribution of the label between unsaturated and saturated fatty acid was approximately 60-40, This ratio was achieved within ten minutes of incubation. In seven day old larvae, most of the label was found in the saturated fraction.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi using Capture Perfect 3.0 on a Canon DR-9050C in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2014-04-02T14:02:53Z (GMT) No. of bitstreams: 1 SunGraceY1983_Redacted.pdf: 1494364 bytes, checksum: 7cbbb0b40d6cc3ae0fe7e19a4903bd37 (MD5)
  • description.provenance : Made available in DSpace on 2014-04-02T23:23:04Z (GMT). No. of bitstreams: 1 SunGraceY1967_Redacted.pdf: 1494364 bytes, checksum: 7cbbb0b40d6cc3ae0fe7e19a4903bd37 (MD5) Previous issue date: 1966-08-04
  • description.provenance : Rejected by Patricia Black(patricia.black@oregonstate.edu), reason: Replace file on 2014-04-02T16:30:11Z (GMT)
  • description.provenance : Submitted by Alex McFerrin (amscannerosu@gmail.com) on 2014-04-02T16:51:18Z No. of bitstreams: 1 SunGraceY1967_Redacted.pdf: 1494364 bytes, checksum: 7cbbb0b40d6cc3ae0fe7e19a4903bd37 (MD5)
  • description.provenance : Submitted by Alex McFerrin (amscannerosu@gmail.com) on 2014-04-02T14:02:22Z No. of bitstreams: 1 SunGraceY1983_Redacted.pdf: 1494364 bytes, checksum: 7cbbb0b40d6cc3ae0fe7e19a4903bd37 (MD5)
  • description.provenance : Rejected by Patricia Black(patricia.black@oregonstate.edu), reason: Remove extra files on 2014-04-02T13:59:25Z (GMT)
  • description.provenance : Approved for entry into archive by Katy Davis(kdscannerosu@gmail.com) on 2014-04-02T16:52:56Z (GMT) No. of bitstreams: 1 SunGraceY1967_Redacted.pdf: 1494364 bytes, checksum: 7cbbb0b40d6cc3ae0fe7e19a4903bd37 (MD5)
  • description.provenance : Approved for entry into archive by Katy Davis(kdscannerosu@gmail.com) on 2014-04-02T23:23:04Z (GMT) No. of bitstreams: 1 SunGraceY1967_Redacted.pdf: 1494364 bytes, checksum: 7cbbb0b40d6cc3ae0fe7e19a4903bd37 (MD5)
  • description.provenance : Submitted by Alex McFerrin (amscannerosu@gmail.com) on 2014-04-01T20:13:07Z No. of bitstreams: 2 SunGraceY1983_Redacted.pdf: 1489629 bytes, checksum: 85ec317aedae243cb385c117dfe6a5cf (MD5) SunGraceY1983_Redacted.pdf: 1489629 bytes, checksum: 85ec317aedae243cb385c117dfe6a5cf (MD5)

Relationships

In Administrative Set:
Last modified: 08/07/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items