Effect of varying levels of vitamin B-6 intake on lymphocyte mitogenic response and vitamin B-6 concentration in human peripheral blood mononuclear cells Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/w66345729

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Two studies were conducted to determine the effect of varying vitamin B-6 (B- 6) status on lymphocyte mitogenic response and pyridoxal 5'-phosphate (PLP) concentration in peripheral blood mononuclear cells (PBMC) in young women. In the first study, women were fed 1 mg/d for the first week and 1.5, 2.1 and 2.7 mg/d during 2 weeks of each of the subsequent 3 experimental periods. Plasma PLP and urinary 4: pyridoxic acid (4-PA) were increased with increasing B-6 intake. B-6 intake > 2.1 mg significantly enhanced lymphocyte proliferation, and non-significantly increased plasma interleukin-2 concentration. Lymphocyte proliferation was significantly correlated with B-6 intake, erythrocyte aminotransferase activity coefficients and plasma PLP. PBMC PLP tended to increase after 2 weeks of 2.7 mg B-6 intake, and was significantly correlated with plasma PLP. In the second study, women consumed their normal diets whose estimated mean dietary B-6 intake was 0.9 mg for 27 d. For the last 20 d, all subjects were given a multivitamin supplement containing 1.8 mg B- 6, and half of the subjects were given an additional 50 mg of B-6 supplement. Plasma PLP and urinary 4-PA were significantly higher in the group with 50 mg B-6, but lymphocyte proliferation did not significantly differ between the groups. After 10 d of supplementation, lymphocyte proliferation was significantly higher than the other time points. A significant increase in PBMC PLP was observed after 3 days and 20 days following 50 mg and multivitamin supplementation only, respectively. After 20 days of supplementation, there was no significant difference of mean PBMC PLP between the groups. PBMC PLP was significantly correlated with plasma PLP, PL and 4-PA. In both studies, no strong relationship was found between PBMC PLP and lymphocyte proliferation. The findings from these studies demonstrate no further benefit of a higher B-6 intake than 2.1 mg on lymphocyte mitogenic response, once the response was significantly enhanced with B-6 intake 0.8 mg higher than the current recommendation. Finally, results from two studies suggest that the current recommendation of vitamin B-6 for young women may not be adequate to maximize lymphocyte mitogenic response and PLP concentration in PBMC.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Academic Affiliation
Non-Academic Affiliation
Subject
Rights Statement
Peer Reviewed
Language
Digitization Specifications
  • File scanned at 300 ppi (Monochrome) using Scamax Scan+ V.1.0.32.10766 on a Scanmax 412CD by InoTec in PDF format. LuraDocument PDF Compressor V.5.8.71.50 used for pdf compression and textual OCR.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2012-01-09T18:14:06Z (GMT) No. of bitstreams: 1 KWAKHOKYUNG2001.pdf: 2487532 bytes, checksum: dfdf05a4f5c4e5cc6c126ddd111b1556 (MD5)
  • description.provenance : Submitted by Erin Clark (ecscannerosu@gmail.com) on 2011-12-05T17:06:39Z No. of bitstreams: 1 KWAKHOKYUNG2001.pdf: 2487532 bytes, checksum: dfdf05a4f5c4e5cc6c126ddd111b1556 (MD5)
  • description.provenance : Approved for entry into archive by Patricia Black(patricia.black@oregonstate.edu) on 2011-12-05T17:24:21Z (GMT) No. of bitstreams: 1 KWAKHOKYUNG2001.pdf: 2487532 bytes, checksum: dfdf05a4f5c4e5cc6c126ddd111b1556 (MD5)
  • description.provenance : Made available in DSpace on 2012-01-09T18:14:06Z (GMT). No. of bitstreams: 1 KWAKHOKYUNG2001.pdf: 2487532 bytes, checksum: dfdf05a4f5c4e5cc6c126ddd111b1556 (MD5) Previous issue date: 2001-07-26

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items