Coordinated regulation of plasma membrane lipids in Saccharomyces cerevisiae Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/wh246w59s

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  • Plasma membranes isolated from a yeast sterol auxotroph (RD5-R) grown on 1,5, and 15 pg m1⁻¹ exogenous concentrations of sterol showed no discontinuity in plots of steady-state fluorescence anisotropy. Liposomes constructed from phospholipid and sterol extracted from RD5-R grown on different sterols indicated exogenously supplied sterol modulated cellular phospholipids such that lipid phase transitions were avoided. Liposomes derived from sterol and phospholipid extracted from the same culture exhibited no lipid phase transitions. However, when phospholipid extracted from a culture grown on a specific sterol was mixed with sterol extracted from a heterologous culture grown on a different sterol to form liposomes, discontinuities were detected in the anisotropy measurements of the liposomes produced. Quantitative analyses revealed that the exogenously supplied sterol regulated specific phospholipid species, fatty acid composition, and sterol to phospholipid ratios in yeast auxotrophs. Analyses of the free sterol, steryl ester, and fatty acids from yeast secretion mutants indicated that the sterol content (free and esterified) remained relatively constant over a growth range of 24 to 34°C. The saturated fatty acid components (C16:0 and C18:0) increased while the unsaturated fatty acids (C16:1 and C18:1) decreased as a function of growth temperature.
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