Transconjugant analysis : the limitations on the use of sequence-specific endonucleases for plasmid identification

Graduate Thesis Or Dissertation

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Citeable URL: https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/wh246w65x

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Attribute Name	Values
Creator	Causey, Stuart Crowell
Abstract	We have used the sequence-specific endonucleases Eco RI, Sma I, Bam HI, Hsu I and Hae III as identification tools in following the conjugal transfer of the well studied R plasmids Sa, R388, RP4 and R6K. Transfers were both intergenus and intragenus. Plasmid fingerprints were generated from both single and combination enzyme digests. The Sa transconjugants yielded plasmids showing consistent fingerprints for each of the respective endonucleases used while the three other R plasmid transconjugants showed both phenotypic changes as well as fingerprint changes.
Resource Type	Masters Thesis
Date Available	2013-10-17T20:20:11+00:00
Date Issued	1978-03-14
Degree Level	Master's
Degree Name	Master of Science (M.S.)
Degree Field	Microbiology
Degree Grantor	Oregon State University
Commencement Year	1978
Advisor	Brown, Lyle R.
Academic Affiliation	Microbiology
Non-Academic Affiliation	Oregon State University. Graduate School
Subject	Microorganisms -- Identification
Rights Statement	Copyright Not Evaluated
Publisher	Oregon State University
Peer Reviewed	No
Language	English [eng]
Digitization Specifications	File scanned at 300 ppi (Monochrome, 8-bit Grayscale) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 5.0 was used for pdf compression and textual OCR.
Replaces	http://hdl.handle.net/1957/43306

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