Transconjugant analysis : the limitations on the use of sequence-specific endonucleases for plasmid identification Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/wh246w65x

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  • We have used the sequence-specific endonucleases Eco RI, Sma I, Bam HI, Hsu I and Hae III as identification tools in following the conjugal transfer of the well studied R plasmids Sa, R388, RP4 and R6K. Transfers were both intergenus and intragenus. Plasmid fingerprints were generated from both single and combination enzyme digests. The Sa transconjugants yielded plasmids showing consistent fingerprints for each of the respective endonucleases used while the three other R plasmid transconjugants showed both phenotypic changes as well as fingerprint changes.
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