Oral and parenteral immunization for the control of Vibrio anguillarum, the etiological agent of vibriosis in salmonid fish Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/wm117r51m

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  • Efficacious vaccines were developed for the control of Vibrio anguillarum, the etiological agent of vibriosis in salmonid fish. These bacterins can be administered either orally or parenterally. It was determined that both formalin-killed lyophilized whole cells and wet-packed whole cells of the organism are effective oral immunogens. Intraperitoneal injection of 0.1 ml containing 2 X 10⁸ formalinkilled bacterial cells suspended in saline and mixed with Freund complete adjuvant is capable of providing protection to fish exposed to natural challenge with V. anguillarum. Oral vaccines prepared from organisms isolated from salmonids in the Pacific Northwest offered greater immunity than bacterins made from European strains when fish receiving them were challenged at Lint Slough, Waldport, Oregon. Several parameters under which the oral vaccine can be effectively used were examined. These investigations revealed that protection is provided to fish vaccinated for 15 days with a ration containing 0.5 mg of the wet whole cell vaccine per gram. Increasing the number of days the vaccine was fed to as many as 45 days did not increase the degree of resistance in immunized fish. Decreased mortality was also not observed in groups of fish fed a diet containing higher concentrations of vaccine. These studies demonstrated that oral immunization of fish can be successfully accomplished at water temperatures ranging from 4 to 21 C. Although agglutinating antibody could not be detected in the serum of orally immunized animals, there is an indication that bactericidal antibodies are formed. Fish parenterally vaccinated produced agglutinating as well as bactericidal antibodies.
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