Relationship of dietary crude protein to factors in uterine secretions and plasma in high producing postpartum dairy cows Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/x633f430v

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  • Eighteen multiparous, high producing (7,836 kg 4% FCM) Holstein dairy cows were assigned randomly to iso- . caloric rations containing either 12 or 23% crude protein (CP) on day 40 postpartum. After a 10-day adaptation period, uterine secretion and blood samples were collected on the day of estrus (day 0-1), day 5 and day 15 of the first estrous cycle after day 50 postpartum and on the day of estrus (day 0-2) of the subsequent estrous cycle. Days to first postpartum estrus, days to first experimental estrus, days from first to second experimental estrus, bodyweights, parity and feed intake did not differ between treatments. Cows fed 23% CP required a 1.9-fold increase. (P<.06) in services per conception (SPC) the year of the experiment as compared to the preceding years, but cows fed 12% CP required the same number of SPC. The ammonia concentration in plasma of cows fed 23% CP was elevated (8.0 ± .5 μg/m1) over cows fed 12% CP (6.3±.3 μg/m1; P<.01). Total protein concentration in the uterine secretions increased 88% from day 0-1 (8.9±1.5 mg/ml) to a peak at day 15 (16.7±1.7 mg/ml; P<.05) and then declined again by day 0-2 (4.6±.7 mg/ml), although it did not change with treatment. Plasma urea concentration was 3.5-fold lower in cows fed 12% CP than 23% CP rations (4.8 ±.3 and 16.8±.5 mg/100 ml, respectively; P<.01). Uterine secretion urea concentration was 2.7-fold higher in cows fed 23% CP (17.2±1.1 vs. 6.4±.7 mg/100 ml). Plasma glucose concentration was lower on days 0-1 and 5 than on days 15 and 0-2 (60.9±.7 and 65.9±1.4 mg/100 ml, respectively; P<.01), but did not differ with treatment. Protein level did not affect actual milk production, % milk fat or 4% FCM. Uterine secretion Ca was higher during the luteal phase (159.0±11.1 ppm), days 5 and 15, than during estrus (81.5±4.0 ppm; P<.01), but did not vary with treatment. Plasma Ca (105.1±1.1 ppm) and Mg (23±.3 ppm) did not change with time or treatment. Cows fed 12% CP had higher Mg in the uterine secretions on day 5 and 15 of the estrous cycle than cows fed 23% CP (66.7±11.1 and 38.6±4.3 ppm, respectively; P<.01). Plasma P was higher (P<.01) in cows fed 23% CP (6.1±.2 mg/100 ml) than in cows fed 12% CP (5.2±.2 mg/100 ml). However, uterine secretion P was higher in the 23% CP group (6.9±1.1 vs. 53 ±.8 mg/100 ml; P<.01), but only on days 5 and 15 of the estrous cycle. Plasma Zn concentration declined from 1.4±.2 to 1.1t.1 ppm from the first to second experimental estrus in cows fed 12% CP, but rose from 1.2±.1 to 1.4±.1 ppm during the same interval in cows fed 23% CP. Uterine secretion Zn declined from .92±.13 to .41±.04 ppm in cows fed 12% CP and from .72±.12 to .52±.10 ppm in cows fed 23% CP from the first to second experimental estrus. Cows fed 23% CP had higher plasma K concentrations than cows fed 12% CP (224±3 and 216±2 ppm, respectively; P=.06). Potassium was higher in the uterine secretions of cows fed 12% CP on days 5 and 15 (1059±124 pg/m1) as compared to cows fed 23% CP (799±61 pg/m1; P<.05). Plasma progesterone was elevated during the luteal phase, but no treatment differences were observed in the peak concentration, although the progesterone level was 19% lower in the cows fed 23% CP on day 15 of the estrous cycle. The interval from estrus to peak progesterone and the number of days the plasma progesterone concentration was <1 ng/ml did not differ with treatment. The decreased fertility without lengthened estrous cycles suggests excess dietary CP affects events preceding implantation, such as ovum or sperm viability, fertilization or early embryonic cleavage and survival. Further, these results indicate protein and its metabolites alter the uterine secretions by decreasing P, Mg, and K the luteal phase of the estrous cycle in cows fed 23% CP relative to cows fed 12% CP.
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