Effects of near-ultraviolet radiation on changes in the biochemical composition of Verticillium albo-atrum Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/x633f437s

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  • This study sought a better understanding of the mechanism of the near-ultraviolet inhibition of microsclerotia and pigment formation in the fungus Verticillium albo-atrum Reinke & Berth. by observing changes which occur in certain major biochemical components: lipids, chitin, RNA, DNA, protein carbohydrates, and pigment. The most important finding was that dark-reared, microsclerotia-producing cultures generally produced about twice as much total lipid as did UV-reared cultures which produced no microsclerotia. At least part of the time, the carbohydrate content of the dark-reared cultures exceeded the carbohydrate content of the UV-reared culture s. The percent apparent protein (measured by the Lowry technique) in the dark-reared cultures declined with age from 25% to 8% by the eleventh day. The apparent protein content of the UV-reared cultures remained between 17-25%. No substantial differences in percent chitin and percent DNA of dark-reared and UV-reared cultures were observed.
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  • File scanned at 300 ppi (Monochrome) using Capture Perfect 3.0.82 on a Canon DR-9080C in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR.
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