Acute and chronic toxicity studies with monochlorobenzene in rainbow trout Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/xk81jn984

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  • The toxicity of chlorobenzene (CB) was investigated in rainbow trout following acute ip administration (1. 0 ml/kg, 1:1 in corn oil) and chronic exposure (2. 1 ppm and 2. 9 ppm) via the water in a continuously flowing system for 15 and 30 days. In the acute study, overt toxicity and hepatotoxicity were monitored over a 96 hour time period. Variables measured to assess toxicity included LD50 determination, weight changes, liver weight to body weight ratios, behavioral changes, alanine aminotransferase activity (GPT), sulfobromophthalein (BSP) retention, total plasma protein concentration and liver histopathology. In the chronic study the same measures of toxicity were followed as well as respiratory rates, oxygen consumption, food consumption, alkaline phosphatase (AP) activity, and serum protein electrophoresis. The 72 hour LD50 of CB in rainbow trout after acute intoxication was 1.8 ml/kg. The toxicant caused behavioral changes in the fish which were consistent with the known anesthetic properties of CB in mammals. Elevations in BSP retention and GPT activity, and histopathology indicated that CB was hepatotoxic in the fish. The LC50 of CB in trout exposed via the water for 96 hours was 4.7 ppm. Chronic exposure of trout to CB resulted in similar behavioral changes as seen in the acute study. Liver toxicity was evident from elevations in GPT activity and decreased A/G ratios of serum proteins after 15 days of exposure. BSP retention and AP activity appeared to be affected by the nutritional status of the trout as much as by the CB treatment. After 30 days of exposure to CB, trout appeared to have adapted to the toxic effects of CB. These results indicate that CB is toxic to rainbow trout after either acute intoxication or chronic exposure via the water. Behavioral and soft organ toxicity of CB seen in this study may compromise the ability of the trout to thrive in its environment.
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