Effect of rumen fluid upon the preservation of bovine spermatozoa Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/xp68kk09z

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  • Experiments were conducted to determine the effect of rumen fluid on live and motile spermatozoa of semen extended in yolk-citrate and stored at 4°C. Preliminary work showed that levels of rumen fluid below 10 percent were beneficial in keeping spermatozoan activity up to 14 days in storage. For more revealing information, 1664 observations for live and motility were made on 16 collections from 4 dairy bulls. Semen was extended in 0.5, 1.0 and 5.0 percent boiled or raw rumen fluid, treated or nontreated with 500 I.U. of penicillin and 500 micrograms of dihydrostreptomycin per ml. of extender. From microscopic evaluations to the ninth day, the results were seen to fall into three definite groups: control, antibiotic treated, and nontreated semen. Higher ratings were observed in the lower levels than in the 5 percent level of rumen fluid (p = .001). Additional 768 observations to determine the effect of argon and oxygen on semen extended in different levels of rumen fluid showed highly significant differences in motility, but no differences in percent alive. For the ninth day rating, rumen fluid at 1.0% or lower levels increased motility by 27 percent in comparison with control. Antibiotics decreased this figure to 11 percent. Oxygen decreased motility by 12 percent; however, at the 5 percent level, oxygen rated 25 percent higher than oxygen without rumen fluid. Argon showed an overall decline in motility of 8 percent. It can be concluded that the activity of bovine spermatozoa can be increased with small amounts of rumen fluid. The detrimental effect of antibiotics on motility (p < .0001) and the nonharmful effect of oxygen on the life of the spermatozoa contradict present belief. The fact that boiling did not affect the beneficial effect of rumen fluid suggests sterile means for using rumen fluid in artificial insemination. Since the action of some substance produced by a rumen microorganism is suspected, the possibility of isolating this microorganism can not be overlooked.
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