Graduate Thesis Or Dissertation
 

Susceptibility of two salmonid fish cell lines to two viruses from fish and two from homothermic animals

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  • This thesis describes the viral susceptibility of two cell cultures established from embryonic tissue of salmonid fish, chinook salmon line CHSE-114 and steelhead trout line STE-137. The viruses used were Oregon sockeye salmon virus (OSV), Sacramento River chinook disease virus (SRCD), Western equine encephalitis virus (WEE) and Newcastle disease virus (NDV). An attempt was made first to determine if the viruses would replicate in the cell lines and secondly to determine if they could be carried by serial propagation and at the same time become adapted to the cultures. The cells were observed microscopically for cytopathic effects caused by the viruses. OSV replicated and was carried by serial propagation in both cell lines, but became adapted only to line CHSE-114. In STE-137 cells, the virus caused a thickening of the nuclear membrane and an increase in the optical density of the nucleoli. The same cytopathic effects were seen in line CHSE-114 only after OSV had become adapted to it. SRCD virus replicated and was readily carried by serial propagation in both STE-137 and CHSE-114 cells, but did not show further adaptation to either line. The cytopathic effects caused by this virus were identical to those caused by OSV. WEE virus replicated in both cell lines at 26°C. It was carried by serial propagation, but did not become further adapted to either line. CHSE-114 cells showed a marked increase in cell degeneration when infected with WEE virus, but no unique cytopathic effect was seen. Two new types of vacuole-like structures were seen in the cytoplasm of STE-137 cells infected with WEE virus. NDV did not replicate in either cell line, nor could it be carried by serial propagation. No cytopathic effects were seen in either cell line.
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