Graduate Thesis Or Dissertation
 

Purification of 2,5-dihydroxyacetanilide epoxidase and mechanism of hydroquinone epoxidases

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  • 2,5-dihydroxyacetanilide epoxidase from Streptomyces LL-C10037 (DHAE I) catalyzes the epoxidation of hydroquinone 4, to form epoxyquinone 7. DHAE I has been purified to homogeneity, with an overall purification factor greater than 23,000. N-terminal and internal amino acid sequences have been obtained from the purified enzyme so that the epoxidase gene may be cloned by using reverse genetics. The epoxidation reaction of DHAE I requires only molecular oxygen and no added cofactors. The detailed chemical mechanism has been elucidated using isotopically labeled oxygen. DHAE I is a dioxygenase belonging to a new and unrecognized class of dioxygenases that are described as "hydroquinone epoxidizing dioxygenases." DHAE I and another epoxidizing enzyme which acts on the same substrate but producing the enantiomeric epoxide, DHAE II from Streptomyces MPP 3051, have had their active sites examined for structural latitude. DHAE II shows more flexibility toward substrate analogs than DHAE I. However, both enzymes are very specific for the correct substrate. From these studies, crude three dimensional models of the two active sites have been constructed.
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