Development, characterization and application of polyclonal antibodies against ovine adipocytes for body fat reduction by passive immunization Public Deposited

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/xw42nc263

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  • Three experiments were conducted (1) to develop and characterize polyclonal antibodies against ovine adipocyte plasma membranes (APM), (2) to evaluate the effect of administering the developed antibodies to lambs on lamb growth and carcass characteristics, and (3) to investigate the possibility of using the developed antibodies to identify antigenic components on ovine APM that are tissue, species and possibly site specific. In Experiment 1, the developed antibodies showed a high rate of reactivity to APM and cross reacted with plasma membranes (PM) from liver, kidney, heart and erythrocytes. Adsorption of antisera with either liver PM or erythrocyte PM for 6 h resulted in a significant reduction in antisera cross-reactivity to liver or erythrocyte PM, respectively, with little effect on its specific reactivity to APM or cross-reactivity with PM of the other tissues tested. The antisera also reacted in different affinities to APM from sheep, pig and rat. The antisera also showed differential binding to APM isolated from different anatomical locations. In Experiment 2, five wether lambs were assigned to either a control or treatment group to study the effect of passive immunization with the antisera immunoglobulin (ASig) on lamb growth and carcass characteristics. Treatment with ASig resulted in a significant (P<.OS) reduction of 45% in wet perirenal fat weight, and of 1.8 and 0.8% in subcutaneous and perirenal lipid content, respectively. ASig immunization also significantly (P<.O5) reduced blood plasma non-esterified fatty acid without adversely affecting blood plasma triglycerides or packed cell volumes (hematocrit). Animals immunized with ASig also showed a significant (P<.OS) reduction in average weight daily weight gain, but this effect was not associated with any adverse effect on efficiency of carcass production. Moreover, passive immunization tended to improve protein accretion and efficiency of nitrogen utilization. In Experiment 3, the developed antisera immunoglobulins recognized three unique ovine APM components, in comparison with plazma membranes from liver, kidney, heart or RBC, with molecular weights of 70, 106 and 110 KD, and showed to react more intensely with APM antigenic components that are in common with PM of the tissues tested. Antisera immunoglobulins recognized just two (44, 46 KD) antigenic components on porcine APM that are shared with those of ovine APM but did not react with any component on APM of either rat or chicken.
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