Graduate Thesis Or Dissertation
 

Ribosomes and protein synthesis in the fat body of Leucophaea maderae (Fab.)

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  • Several procedures for isolating ribosomes from the fat body of female cockroaches are described and evaluated. The addition of deoxycholate or Triton X-100 to the homogenizing medium rather than to the postmitochondrial supernatant was an important factor in improving the yield of ribosomes. The fat body ribosomes were characterized and found to behave differently than ribosomes isolated from the tissues of other animals. The majority of the particles were heavy aggregates, and the ribosomes appeared to be relatively stable to RNase treatment. Increasing the KC1 concentration from 25 mM to 250 mM resulted in some dissociation of heavy aggregates. The principal properties of the cell-free protein synthesizing system were also studied. The system did not require GTP and ATP-generating systems. RNase caused an unexpected stimulation of incorporation of amino acid into protein.
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